Currently 80% of biological samples are transparent. Traditional bright-field microscopes have low contrast, loss of three-dimensional topography information, and inability to automatically adjust focus. Digital holographic microscopy technology: the use of object light and The hologram formed by the interference of the reference light records the sample information, and the amplitude/phase information of the sample is reproduced numerically. The phase imaging and optical microscopy technology are combined to quantitatively measure the spatial phase change caused by the sample to be tested. Labeling, less damage to samples, high contrast imaging of transparent samples, compared with traditional fluorescence imaging (phototoxicity, fluorescence quenching), innovative 4D imaging technology, non-toxic, continuous imaging; compared with traditional digital holographic microscope (Bulky and poor anti-interference ability), it has been miniaturized and can be put into the cell culture room, and continuous and real-time observation of cell growth becomes possible.

• Can realize 4D imaging of transparent samples
  

  Using phase imaging, real-time, high-contrast, three-dimensional imaging of transparent samples can be performed
  Spatial resolution: 260 nm/500 nm/1000 nm; Temporal resolution : 20 ms

• Non-invasive, auto-focusing
  

  Without fluorescent labeling, long-term continuous observation of living cells is possible
  

• Miniaturization
  

  Can be placed directly in the cell culture box, observe the growth of living cells at 37oC+5%CO2
  

• High sensitivity
  

  Designed a point diffraction holographic optical path, achieving international leading imaging Sensitivity
  

  The optical path difference fluctuates by 0.012λ (6.4 nm) within 3 hours of the device